Readily deployable technology for reproducible cryogenic sample time-resolved electron microscopy (cryoTREM)

SUMMARY This advanced technology grant focuses on enhancing cryogenic sample time-resolved electron microscopy (cryoTREM) through the development of a patterned LED/LCD-based illumination system capable of capturing multiple temporal states on a single EM grid with 4 orders of magnitude time range from 1 to 10,000 milliseconds. This system will be developed and deployed in producing time-resolved data sets for several different biological systems with intermediate states with varying degree of conformational magnitude. The resultant data will be integrated with a variety of data processing methods to determine ensembles of time-resolved structures, which can be leveraged to understand molecular mechanisms. These time-resolved EM experiments will be conducted on multiple biological systems and processes including ligand-gated ion channels, a lipid transferase, the V- ATPase, and synaptic vesicle fusion. Project Number: 1R01GM160508-01 | Fiscal Year: 2025 | NIH Institute/Center: National Institute of General Medical Sciences (NIGMS) | Principal Investigator: Michael Stowell | Institution: UNIVERSITY OF COLORADO, Boulder, CO | Award Amount: $2,113,889 | Activity Code: R01 | Study Section: Macromolecular Structure and Function C Study Section[MSFC] View on NIH RePORTER: https://reporter.nih.gov/project-details/11207742